Carbohydrate-binding modules targeting branched polysaccharides: overcoming side-chain recalcitrance in a non-catalytic approach

Abstract Extensive decoration of backbones is a major factor resulting in resistance enzymatic conversion hemicellulose and other branched polysaccharides. Employing debranching enzymes the main strategy to overcome this kind recalcitrance at present. A carbohydrate-binding module (CBM) contiguous amino acid sequence that can promote binding various carbohydrates, thereby facilitating hydrolysis. According previous studies, CBMs be classified into four types based on their preference ligand type, where Type III IV prefer polysaccharides than linear thus are able specifically enhance hydrolysis substrates containing side chains. With role dominating substrates, could represent non-catalytic approach overcoming side-chain recalcitrance.